Pseudopterogorgia sp. extract were fractionated on silica gel to crop fractions: F-1 (pseudopterosins PsQ, PsS and PsU) and F-2 (amphilectosins A and B, PsG, PsK, PsP and PsT and seco-pseudopterosins seco-PsJ and seco-PsK) from chemotype 1, and F-3 (elisabethatrienol, 10-acetoxy-9-hydroxy- and 9-acetoxy-10-hydroxy-amphilecta-8,10,12,14-tetraenes (interconverting mixture) and amphilecta-8(13),11,14-triene-9,10-dione) from chemotype 2. By application anterior RP-HPLC and spectroscopic means, we acquired the authentic PsG, PsK, PsP, PsQ, PsS, PsT, PsU, seco-PsK and the interconverting admixture of non-glycosylated diterpenes (IMNGD). The anti-inflammatory backdrop of extracts and fractions were evaluated application in vivo archetypal "12-O-tetradecanoyl-phorbol-acetate (TPA)-induced abrasion ear oedema". The activities of authentic compounds and of the IMNGD were evaluated application in vitro assays myeloperoxidase (MPO) absolution (by animal polymorphonuclear neutrophils (PMNs)), nitric oxide absolution (by J-774 cells) and scavenger action on NO.
In the in vivo anti-inflammatory assay, extracts and F-3 showed low inhibition levels of deepening compared to indomethacin, F-1 and F-2. Additionally, we evaluated the MPO absolution to the deepening site, and begin a apparent inhibition of MPO levels by all extracts and fractions, even above to the inhibition apparent by indomethacin.
Furthermore, in the MPO in vitro assay, IMNGD, PsQ, PsS, PsT and PsU, apparent college inhibition levels compared to dexamethasone and indomethacin. In the NO absolution in vitro, IMNGD, PsP and PsT were the a lot of almighty treatments. Finally, because the PsG, PsP and seco-PsK did not display any NO scavenger activity, they should arrest the inducible Nitric Oxide Synthase (iNOS) or added routes that access this enzyme. Alternatively, PsQ, PsS, and PsU did appearance scavenger activity.
All after-effects presented accord to authenticate that the compounds abandoned in this plan from Pseudopterogorgia elisabethae are able molecules with an absorbing anti-inflammatory action profile. Additionally, the after-effects acquired could accommodate basic insights appear their structure-activity relationship.
Large colonies, composed of abundant capital branches with abate pinnately abiding branchlets, feather-like plumose; as ample as 1 m in height. Branchlets long, hardly flattened, tapered, and 1.5-2.5 mm in diameter. Apertures baby and oval, abiding in 2-3 rows on anniversary of the attenuated edges of the branches. It produces copious amounts of mucus.
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