What is Rhodamine 6G?

Rhodamine 6G is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
Rhodamine 6G is also used as a laser dye, or gain medium, in dye lasers, and is pumped by the 2nd (532 nm) harmonic from an Nd:YAG laser or nitrogen laser. The dye has a remarkably high photostability, high fluorescence quantum yield (0.95), low cost, and its lasing range has close proximity to its absorption maximum (approximately 530 nm). The lasing range of the dye is 555 to 585 nm with a maximum at 566 nm.
Rhodamine 6G usually comes in three different forms. Rhodamine 6G chloride is a bronze/red powder with the chemical formula C27H29ClN2O3. Although highly soluble, this formulation is very corrosive to all metals except stainless steel. Other formulation are less soluble, but also less corrosive. Rhodamine 6G Perchlorate, (C27H29ClN2O7), comes in the form of red crystals, while rhodamine 6G tetrafluoroborate, (C27H29BF4N2O3), appears as maroon crystals.
Rhodamine is a family of related chemical compounds, fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are used as a dye and as a dye laser gain medium. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
Rhodamine dyes are generally toxic, and are soluble in water, methanol and ethanol.
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