Applications of Pyranine

Pyranine is a hydrophilic, pH-sensitive fluorescent dye from the group of chemicals known as arylsulfonates. Pyranine is soluble in water and has applications as a coloring agent, biological stain, optical detecting reagent, and a pH indicator. One example would be the measurement of intracellular pH. Pyranine is also found in highlighters and soaps
APPLICATIONS of Pyranine
Solvent dyes are insoluble in water but soluble in nonpolar organic solvents and fats. It is usually used as a solution in an organic solvent. Azo structure solvent dyes are often responsible yellow-red color whereas anthraquinone structure solvent dyes tend to be green-blue. Some other structures for solvent dyes include heterocyclic rings, metal complex, pyrazolone, xanthene, and ketoamine. Solvent dyes do not ionise. Solvent dye, undergoing molecular rearrangement, is dissoluted in the substrate. It colours and binds to the lipids resulting in showing colored regions. In industrial field, it is used in coloring oils, waxes, greases, fats, hydrocarbons derivatives, polishes, oily insecticides, and acrylic emulsions. Pyranine (sulfonated hydroxy pyrene trisodium salt) is soluble in water and has applications in the field of :
Coloring agent for drug and cosmetics
Biological stain
Optical detecting reagent for bile acids
Flash-lamp laser dye
pH indicator for physiological range
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